Fig. 5

The elevated astrocytes and microglia/macrophages accumulation in TG and TNC of mTBI mice was attenuated by MJN110 in a cannabinoid receptor dependent manner. On day 14 post-injury, the Iba1-positive immunostaining was significantly increased in the TG and TNC (A; arrows in the merged images point to macrophages) of the mTBI mice. MJN treatment remarkably reduced positive Iba1 cells in both regions (B, C). Co-administration of AM281 significantly reversed the MJN inhibitory effect on macrophages accumulation in the TG (B), but not in the TNC (C). Conversely, addition of AM630 significantly reversed the MJN effect in TNC (C), but not in TG (B). Both CB1 and CB2 receptor antagonists reversed MJN effect on GFAP accumulation in the TNC (D, E). *, p < 0.05, **, p < 0.01, and ***, p < 0.001 (n = 8/group). Scale bar = 50 μm