Fig. 6

Attenuation of MJN110 on the expression of CGRP in both TG and TNC of the mTBI mice was regulated by cannabinoid signaling pathways. On day 14 post-injury, the CGRP-positive immunostaining was significantly increased in the TG and TNC of the mTBI mice, and MJN treatment remarkably attenuated CGRP immunostaining in both regions (A). In the TG of mTBI mice, The CB2R antagonist AM630 significantly counteracted the downregulation of CGRP by MJN (B). In the TNC of mTBI mice, animals treated with MJN and the CB1R antagonist AM281 had significantly increased CGRP positive immunostaining compared to the MJN alone treated group (C). *, p < 0.05, **, p < 0.01, and ***, p < 0.001 (n = 8/group). Scale bar = 50 μm