Fig. 6

tONS treatment alleviated CGRP expressions in the PAG of IS rats. (A) Representative images of immunofluorescence staining showing CGRP-labeled cells (red) in the PAG (left). The numbers of CGRP-positive neurons in the PAG increased significantly after repeated IS injections compared with the NS group (right). Significantly reduced cell numbers were observed in the IS + True group compared with the sham tONS treatment (n = 6 rats/group, right). (B) Typical western blot images of CGRP from the PAG (left). The band intensity ratio of CGRP in the PAG increased significantly after repeated IS injections compared with the NS group (right). Significantly reduced ratios were observed in the IS + True group compared with the sham tONS treatment (n = 5 rats/group, right). ## P < 0.01, IS + Sham vs. NS + Sham; * P < 0.05, ** P < 0.01, IS + True vs. IS + Sham; error bars represent SEM