Fig. 1

Acute nitroglycerin (NTG)-induced cephalic allodynia is blocked by acute MAGL inhibition in male and female C57BL6/J mice. (A) Schematic of paradigm includes measuring baseline cephalic thresholds, injecting ABD-1970 (10 mg/kg PO), methylcellulose vehicle control (PO), or olcegepant (1 mg/kg IP) 4 h before von Frey testing (teal circle), injecting nitroglycerin (10 mg/kg IP) or saline vehicle 2 h before testing (open circle), and injecting sumatriptan (0.6 mg/kg IP) 45 min before testing (filled in orange circle). (B) Basal responses and (C) post-drug test responses are shown (n = 8/group). 1-way ANOVA analysis, significant effect of treatment (p < 0.001). Holm-Sidak multiple comparisons: **p < 0.01, ***p < 0.001 relative to NTG-Veh, ###p < 0.001 relative to Veh-Veh. Female animals indicated with open circles. All data are presented as mean ± SEM