Fig. 4

MAGL is highly expressed in the TG and TNC, with higher expression in Rbfox3/NeuN expressing cells. (A) Representative images of fluorescent in situ hybridization. Chevron indicates a cell expressing MAGL and Rbfox3, arrow indicates a cell expressing CB₁ and Rbfox3 transcripts. Percentage of total cells expressing transcripts stained with DAPI in (B) TG and (C) TNC. Percentage of total Rbfox3 + cells expressing MAGL and/or CB₁ transcript in the (C) TG and (E) TNC. Percentage of total Rbfox3 negative cells expressing MAGL and/or CB₁ transcript in the (D) TG and (F) TNC. Data are the average from 5 mice, at least four 20x images/mouse were quantified using HALO software. Data represented as mean ± SEM. Females are represented with clear symbols