Fig. 5

Inhibitor activity of AKU-005 against mouse and human MAGL and ABHD6 tested in a glycerol assay. A Dose-dependent inhibition by AKU-005 on human (h) and mouse (m) MAGL enzyme. B Dose-dependent inhibition by AKU-005 on human and mouse ABHD6 enzymes. The enzymes were overexpressed in HEK293 cells and lysates were processed as described in Methods. The data are from at least three independent experiments performed in duplicate. C Table with Log IC₅₀ and IC₅₀ values of AKU-005 for inhibiting human (h) and mouse (m) MAGL and ABHD6. The results were obtained by testing AKU-005 in MAGL- and ABHD6-overexpressing HEK293 cell preparations in a sensitive glycerol assay, as described in methods. An asterisk (*) denotes a statistically significant difference between the IC₅₀ values of hMAGL and mMAGL (two-way paired t-test, p = 0.0012). The data are from at least 3 independent experiments