Fig. 6

CSD calcium transients induced by KCl in rat and mouse cortical slices. Slices were incubated for 4 h with DMSO (control) or AKU-005 (AKU) 100 nM (pretreatment), each slice was then incubated for 20 min with fluorescent probe Fluo- 4, followed by 20 min resting condition in aCSF at room temperature. After this, slices were tested in their response to KCl 100 mM. Protocols applied are described in detail in Material and Methods section. A and B Fluorescence snapshot of rat and mouse cortical slices at 0, 2, 6, 15 s post KCl 100 mM perfusion. Scale bar is 1 mm. C Rat cortical slices, calcium fluorescence time series of DMSO pre-incubation control condition. Black trace is representative of the statistics, all other traces are shown in grey. D Rat cortical slices, calcium fluorescence time series of AKU 100 nM pre-incubation condition. Black trace is representative of the statistics, all other traces are shown in grey. E Mouse cortical slices, calcium fluorescence time series of DMSO pre-incubation control condition. Black trace is representative of the statistic, all other traces are shown in grey. F Mouse cortical slices, calcium fluorescence time series of AKU 100 nM pre-incubation condition. Black trace is representative of the statistics, all other traces are shown in grey. G Statistical comparison of intensity of fluorescence (ΔF/F0(%)) of KCl-triggered CSD in rat cortical slices. CSD fluorescence intensity was reduced upon 4 h AKU pretreatment (n = 18 in CSD and n = 11 in AKU CSD, p = 0.015, Mann–Whitney test). H Statistical comparison of the area under the curve (AUC) of KCl-triggered CSD in rat cortical slices. AKU-005 pretreatment significantly decreases the CSD AUC (p = 0.007, Mann–Whitney test). I Statistical comparison of intensity of fluorescence (ΔF/F0(%)) of KCl-triggered CSD in mouse cortical slices. Changes in CSD fluorescence intensity were not detected after AKU preincubation (n = 13). L Statistical comparison of the AUC of KCl-triggered CSD in mouse cortical slices. AKU-005 pretreatment reduced the CSD AUC (p = 0.040, Unpaired t-test)