Fig. 1

CGRP and SP release from the dura mater in same experiments, 10 days (A, B) or 30 days (C, D) after injection of fremanezumab or isotype control antibody (Control Ab) in female (F) and male (M) animals. Basal release is determined after periods of 5 and 10 min in buffer (SIF) followed by the release stimulated with 500 nM capsaicin (Cap) and by a post-stimulation period in SIF. Each data point represents the mean ± SEM of 6 experiments. Significant differences between basal (SIF2) and stimulated release (**, p < 0.001) and between the two antibodies in stimulated release (##, p < 0.001; #, p < 0.01)