Fig. 6

Nos2 was upregulated in ApoE4-TR mice. (A) Heatmap showing the changes in gene expression of ApoE4-TR mice compared to ApoE3-TR mice after SNI. Each row represents a gene, and each column represents a sample. Red indicates higher expression levels, while blue indicates lower expression levels. (B) The volcano plot shows significantly altered genes in ApoE4-TR mice after SNI. Blue dots represent downregulated genes, red dots represent upregulated genes, and black dots represent genes with no significant changes. (C) The GO chord plot displays the relationship between the selected pathways and their corresponding genes. (D) Significantly enriched GO items in biological processes (BP), cellular components (CC), and molecular functions (MF). (E) Bar chart showing the KEGG pathways analysis for differentially expressed genes. (F) The Venn diagram shows the shared and unique genes among the three groups of differentially expressed genes, lipid metabolism gene set, and oxidative stress gene set. (G) Illustration of the clusters in the PPI network. (H) The top 20 genes ranked after filtering the PPI interaction network. (I) RT-qPCR analysis of the top six gene expressions in the dorsal horn of ApoE3-TR and ApoE4-TR mice 14 days after SNI, including sham-operated controls. Data were normalized to the ApoE3-TR Sham group and presented as “fold change” in the graphs. n = 5 independent biological samples. Gene expression was analyzed using a one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01 and ****P < 0.0001