Fig. 1

Behavioral assessments following mTBI in mice. a A schematic representation of the experimental procedure, with an illustration depicting the position of mTBI hits on the dorsum of the head and a timeline depicting behavioral measurement and sample collection. b Increased righting reflex, a measure of comatose state, in mice following mTBI in the bregma or pre-bregma positions. c Depending on localization of injury, mTBI induced differential peri-orbital sensitivity or mechanical threshold measured by von Frey filaments in the periorbital region. d Increased grimace score in mTBI mice observed 5 days after the last mTBI hit on bregma position. *, p < 0.05, **, p < 0.01, ***, p < 0.001 and ****, p < 0.0001. (n = 5–6/group, Mean ± SEM)